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cd36 blocking antibody sr bi  (Novus Biologicals)


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    Novus Biologicals cd36 blocking antibody sr bi
    PEMs and liver tissues were collected from Mst1 +/+ and Mst1 ΔM/ΔM mice infected with 20 ± 5 cercariae and sacrificed at week 12. (A) RNA-seq analysis was performed on differentially expressed genes (DEG) in PEMs from infected Mst1 +/+ and Mst1 ΔM/ΔM mice control Mst1 +/+ and Mst1 ΔM/ΔM mice (n = 3). DEGs were identified in all pairwise comparisons and showed a 2-fold change in expression with an adjusted p- value of 0.05. (B) Some DEGs were verified by qPCR in PEMs from infected mice. (C) Liver sections from infected mice were subjected to HE staining (leftmost panel) and dual immunofluorescence for <t>CD36</t> and F4/80 (2–5 panels on the right side) in. High-magnification images of co-staining for F4/80 and CD36 cells were shown in the right most panel. The fluorescence intensity of CD36 overlaid with F4/80 + cells was analyzed using ImageJ. Data are shown as mean ± SEM of three different liver sections in each mouse (n = 9, data pooled from 3 independent experiments, 3 mice per group for each experiment. Bar of the left five panels = 100 μm, bar of the far right panel = 50 μm).All p values were determined by Student’s t -test. * P < 0.05, ** P < 0.01.
    Cd36 Blocking Antibody Sr Bi, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36+blocking+antibody+sr-bi/SR-BI+Antibody+-+BSA+Free/pmc11785294-181-1-9
    Average 93 stars, based on 136 article reviews
    cd36 blocking antibody sr bi - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Macrophage MST1 protects against schistosomiasis-induced liver fibrosis by promoting the PPARγ-CD36 pathway and suppressing NF-κB signaling"

    Article Title: Macrophage MST1 protects against schistosomiasis-induced liver fibrosis by promoting the PPARγ-CD36 pathway and suppressing NF-κB signaling

    Journal: PLOS Pathogens

    doi: 10.1371/journal.ppat.1012790

    PEMs and liver tissues were collected from Mst1 +/+ and Mst1 ΔM/ΔM mice infected with 20 ± 5 cercariae and sacrificed at week 12. (A) RNA-seq analysis was performed on differentially expressed genes (DEG) in PEMs from infected Mst1 +/+ and Mst1 ΔM/ΔM mice control Mst1 +/+ and Mst1 ΔM/ΔM mice (n = 3). DEGs were identified in all pairwise comparisons and showed a 2-fold change in expression with an adjusted p- value of 0.05. (B) Some DEGs were verified by qPCR in PEMs from infected mice. (C) Liver sections from infected mice were subjected to HE staining (leftmost panel) and dual immunofluorescence for CD36 and F4/80 (2–5 panels on the right side) in. High-magnification images of co-staining for F4/80 and CD36 cells were shown in the right most panel. The fluorescence intensity of CD36 overlaid with F4/80 + cells was analyzed using ImageJ. Data are shown as mean ± SEM of three different liver sections in each mouse (n = 9, data pooled from 3 independent experiments, 3 mice per group for each experiment. Bar of the left five panels = 100 μm, bar of the far right panel = 50 μm).All p values were determined by Student’s t -test. * P < 0.05, ** P < 0.01.
    Figure Legend Snippet: PEMs and liver tissues were collected from Mst1 +/+ and Mst1 ΔM/ΔM mice infected with 20 ± 5 cercariae and sacrificed at week 12. (A) RNA-seq analysis was performed on differentially expressed genes (DEG) in PEMs from infected Mst1 +/+ and Mst1 ΔM/ΔM mice control Mst1 +/+ and Mst1 ΔM/ΔM mice (n = 3). DEGs were identified in all pairwise comparisons and showed a 2-fold change in expression with an adjusted p- value of 0.05. (B) Some DEGs were verified by qPCR in PEMs from infected mice. (C) Liver sections from infected mice were subjected to HE staining (leftmost panel) and dual immunofluorescence for CD36 and F4/80 (2–5 panels on the right side) in. High-magnification images of co-staining for F4/80 and CD36 cells were shown in the right most panel. The fluorescence intensity of CD36 overlaid with F4/80 + cells was analyzed using ImageJ. Data are shown as mean ± SEM of three different liver sections in each mouse (n = 9, data pooled from 3 independent experiments, 3 mice per group for each experiment. Bar of the left five panels = 100 μm, bar of the far right panel = 50 μm).All p values were determined by Student’s t -test. * P < 0.05, ** P < 0.01.

    Techniques Used: Infection, RNA Sequencing, Control, Expressing, Staining, Immunofluorescence, Fluorescence

    WT or Mst1 deficiency peritoneal exudate macrophages (PEMs) were stimulated with or without SEA (50μg/ml) for 24h, followed by incubation with FITC-Dextran for 2 h. (A) Flow cytometry analysis of the proportion of CD36 + cells. Isotype-matched control IgG staining (----); anti-mouse CD36 staining (——, no SEA;——, with SEA) The representative percentage of CD36 positive cells are shown in the left panels. Gating strategies are shown in the left panels. (B) Flow cytometry analysis of the proportion of PEMs engulfing FITC-dextran, with analysis of mean fluorescence intensity (MFI) in engulfed PEMs. (C) Immunofluorescence assay of PEMs engulfing FITC-dextran, with representative images from three separate cell isolates (magnification×200) and percentages of FITC + in F4/80 + PEMs shown. Ten viewing fields were analyzed in each experiment(Bar of the left three panels = 40 μm, bar of the far right panel = 20 μm). (D, E) Changes in gene expression in PEMs after the uptake of FITC-dextran (37°C, 2h). (F, G) SEA cultured WT PEMs were pretreated with isotype control (clone EPR25A, ab172730) and or CD36 function-blocking antibody (clone EPR22509-40, ab252922), followed by incubation with FITC-Dextran (2 h, 37°C), and subjected to immunofluorescent staining analysis. Representative images were shown (magnification×200) on left panels. Percentages of coimmunostaining of CD36 + and FITC + PEMs were shown on the right side (Bar of the left three panels = 40 μm, bar of the far right panel = 20 μm) (F). mRNA expressions of indicated genes were detected by qPCR (G). Data are shown as mean ± SEM (n = 3, three samples consisting of pooled PEMs of two or three mice from representative of three independent experiments). Statistical significance was determined by student t test (A-F) and one-way ANOVA analysis (G). * P < 0.05, ** P < 0.005, *** P < 0.001.
    Figure Legend Snippet: WT or Mst1 deficiency peritoneal exudate macrophages (PEMs) were stimulated with or without SEA (50μg/ml) for 24h, followed by incubation with FITC-Dextran for 2 h. (A) Flow cytometry analysis of the proportion of CD36 + cells. Isotype-matched control IgG staining (----); anti-mouse CD36 staining (——, no SEA;——, with SEA) The representative percentage of CD36 positive cells are shown in the left panels. Gating strategies are shown in the left panels. (B) Flow cytometry analysis of the proportion of PEMs engulfing FITC-dextran, with analysis of mean fluorescence intensity (MFI) in engulfed PEMs. (C) Immunofluorescence assay of PEMs engulfing FITC-dextran, with representative images from three separate cell isolates (magnification×200) and percentages of FITC + in F4/80 + PEMs shown. Ten viewing fields were analyzed in each experiment(Bar of the left three panels = 40 μm, bar of the far right panel = 20 μm). (D, E) Changes in gene expression in PEMs after the uptake of FITC-dextran (37°C, 2h). (F, G) SEA cultured WT PEMs were pretreated with isotype control (clone EPR25A, ab172730) and or CD36 function-blocking antibody (clone EPR22509-40, ab252922), followed by incubation with FITC-Dextran (2 h, 37°C), and subjected to immunofluorescent staining analysis. Representative images were shown (magnification×200) on left panels. Percentages of coimmunostaining of CD36 + and FITC + PEMs were shown on the right side (Bar of the left three panels = 40 μm, bar of the far right panel = 20 μm) (F). mRNA expressions of indicated genes were detected by qPCR (G). Data are shown as mean ± SEM (n = 3, three samples consisting of pooled PEMs of two or three mice from representative of three independent experiments). Statistical significance was determined by student t test (A-F) and one-way ANOVA analysis (G). * P < 0.05, ** P < 0.005, *** P < 0.001.

    Techniques Used: Incubation, Flow Cytometry, Control, Staining, Fluorescence, Immunofluorescence, Gene Expression, Cell Culture, Blocking Assay

    (A) Pathway analysis of the RNA-Seq data comparing PEMs from Mst1 +/+ and Mst1 ΔM/ΔM mice infected by S . japonicum for 12 weeks. (B) Immunofluorescence assay of SEA (24 h, 50μg/ml) or control cultured WT and MST1- deficiency PEMs for CD36 and PPARγ. Images were collected with a confocal microscope and CD36 intensity was quantified using ImageJ. Data are shown as mean ± SEM (n = 3, three samples consisting of pooled PEMs of two or three mice from representative of three independent experiments. Bar = 10 μm). (C) Immunofluorescence assay for CD36 (green), PPARγ (red) in RAW264.7 cells stimulated with or without SEA (24 h, 50μg/ml), followed by XMU-MP-1 incubation (3μM, 6h). Images were taken with a confocal microscope. Percent nuclear PPARγ fluorescence and CD36 intensity in macrophages with PPARγ localization were analyzed using ImageJ. Data from at least three independent experiments, with 10 viewing fields were analyzed in each (Bar of the left four panels = 10 μm, bar of the far right panel = 5 μm). (D, E) Immunoblot analysis of phosphorylated (p-)PPARγ and total PPARγ (left and middle) and relative Cd36 mRNA (right) in RAW264.7 cells treated with SEA ± XMU-MP-1(D) and in liver tissues from Mst1 +/+ and Mst1 ΔM/ΔM mice infected and control mice (E). Values are means ± SEM (n = 3, from representative of three independent experiments). Statistical significance was determined by Mann-Whitney U-test (B), one way ANOVA analysis (C) and Student’s t test (D-E). * P < 0. 05, ** P < 0. 01.
    Figure Legend Snippet: (A) Pathway analysis of the RNA-Seq data comparing PEMs from Mst1 +/+ and Mst1 ΔM/ΔM mice infected by S . japonicum for 12 weeks. (B) Immunofluorescence assay of SEA (24 h, 50μg/ml) or control cultured WT and MST1- deficiency PEMs for CD36 and PPARγ. Images were collected with a confocal microscope and CD36 intensity was quantified using ImageJ. Data are shown as mean ± SEM (n = 3, three samples consisting of pooled PEMs of two or three mice from representative of three independent experiments. Bar = 10 μm). (C) Immunofluorescence assay for CD36 (green), PPARγ (red) in RAW264.7 cells stimulated with or without SEA (24 h, 50μg/ml), followed by XMU-MP-1 incubation (3μM, 6h). Images were taken with a confocal microscope. Percent nuclear PPARγ fluorescence and CD36 intensity in macrophages with PPARγ localization were analyzed using ImageJ. Data from at least three independent experiments, with 10 viewing fields were analyzed in each (Bar of the left four panels = 10 μm, bar of the far right panel = 5 μm). (D, E) Immunoblot analysis of phosphorylated (p-)PPARγ and total PPARγ (left and middle) and relative Cd36 mRNA (right) in RAW264.7 cells treated with SEA ± XMU-MP-1(D) and in liver tissues from Mst1 +/+ and Mst1 ΔM/ΔM mice infected and control mice (E). Values are means ± SEM (n = 3, from representative of three independent experiments). Statistical significance was determined by Mann-Whitney U-test (B), one way ANOVA analysis (C) and Student’s t test (D-E). * P < 0. 05, ** P < 0. 01.

    Techniques Used: RNA Sequencing, Infection, Immunofluorescence, Control, Cell Culture, Microscopy, Incubation, Fluorescence, Western Blot, MANN-WHITNEY

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    Membrane:

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    Article Snippet: Protein concentrations were determined by the Lowry assay, equal amounts of protein pooled from three to four samples, and proteins separated on a Bio-Rad 4-15% Tris-HCl Ready gel (Bio-Rad Laboratories, Hercules, CA) under denaturing conditions. .. Proteins were transferred to a polyvinylidene difluoride membrane that was blocked in Tris-buffered saline containing 0.1% Tween and 5% dry milk for 1 h. After blocking, blots were incubated with chicken anti-LDL receptor IgG (Novus Biologicals, Littleton, CO) or rabbit anti-SR-BI receptor IgG (Novus Biologicals). .. After 1 h at 37°C, the membranes were washed and then incubated with the appropriate secondary antibody conjugated with peroxidase (rabbit anti-chicken IgG and donkey anti-rabbit; GE Healthcare).

    Article Title: Transport of maternal cholesterol to the fetus is affected by maternal plasma cholesterol concentrations in the Golden Syrian hamster
    Article Snippet: Protein concentrations were determined by the Lowry assay, equal amounts of protein pooled from three to four samples, and proteins separated on a Bio-Rad 4-15% Tris-HCl Ready gel (Bio-Rad Laboratories, Hercules, CA) under denaturing conditions. .. Proteins were transferred to a polyvinylidene difluoride membrane that was blocked in Tris-buffered saline containing 0.1% Tween and 5% dry milk for 1 h. After blocking, blots were incubated with chicken anti-LDL receptor IgG (Novus Biologicals, Littleton, CO) or rabbit anti-SR-BI receptor IgG (Novus Biologicals). .. After 1 h at 37°C, the membranes were washed and then incubated with the appropriate secondary antibody conjugated with peroxidase (rabbit anti-chicken IgG and donkey anti-rabbit; GE Healthcare).

    Saline:

    Article Title: Transport of maternal cholesterol to the fetus is affected by maternal plasma cholesterol concentrations in the Golden Syrian hamster
    Article Snippet: Protein concentrations were determined by the Lowry assay, equal amounts of protein pooled from three to four samples, and proteins separated on a Bio-Rad 4-15% Tris-HCl Ready gel (Bio-Rad Laboratories, Hercules, CA) under denaturing conditions. .. Proteins were transferred to a polyvinylidene difluoride membrane that was blocked in Tris-buffered saline containing 0.1% Tween and 5% dry milk for 1 h. After blocking, blots were incubated with chicken anti-LDL receptor IgG (Novus Biologicals, Littleton, CO) or rabbit anti-SR-BI receptor IgG (Novus Biologicals). .. After 1 h at 37°C, the membranes were washed and then incubated with the appropriate secondary antibody conjugated with peroxidase (rabbit anti-chicken IgG and donkey anti-rabbit; GE Healthcare).

    Article Title: Transport of maternal cholesterol to the fetus is affected by maternal plasma cholesterol concentrations in the Golden Syrian hamster
    Article Snippet: Protein concentrations were determined by the Lowry assay, equal amounts of protein pooled from three to four samples, and proteins separated on a Bio-Rad 4-15% Tris-HCl Ready gel (Bio-Rad Laboratories, Hercules, CA) under denaturing conditions. .. Proteins were transferred to a polyvinylidene difluoride membrane that was blocked in Tris-buffered saline containing 0.1% Tween and 5% dry milk for 1 h. After blocking, blots were incubated with chicken anti-LDL receptor IgG (Novus Biologicals, Littleton, CO) or rabbit anti-SR-BI receptor IgG (Novus Biologicals). .. After 1 h at 37°C, the membranes were washed and then incubated with the appropriate secondary antibody conjugated with peroxidase (rabbit anti-chicken IgG and donkey anti-rabbit; GE Healthcare).

    Blocking Assay:

    Article Title: Transport of maternal cholesterol to the fetus is affected by maternal plasma cholesterol concentrations in the Golden Syrian hamster
    Article Snippet: Protein concentrations were determined by the Lowry assay, equal amounts of protein pooled from three to four samples, and proteins separated on a Bio-Rad 4-15% Tris-HCl Ready gel (Bio-Rad Laboratories, Hercules, CA) under denaturing conditions. .. Proteins were transferred to a polyvinylidene difluoride membrane that was blocked in Tris-buffered saline containing 0.1% Tween and 5% dry milk for 1 h. After blocking, blots were incubated with chicken anti-LDL receptor IgG (Novus Biologicals, Littleton, CO) or rabbit anti-SR-BI receptor IgG (Novus Biologicals). .. After 1 h at 37°C, the membranes were washed and then incubated with the appropriate secondary antibody conjugated with peroxidase (rabbit anti-chicken IgG and donkey anti-rabbit; GE Healthcare).

    Article Title: Macrophage MST1 protects against schistosomiasis-induced liver fibrosis by promoting the PPARγ-CD36 pathway and suppressing NF-κB signaling.
    Article Snippet: MST1 kinase inhibitor XMU-XP-1 (SML2233) was purchased from Sigma-Aldrich (St. Louis, MO, USA). .. The CD36 blocking antibody SR-BI (NB400-104) was purchased from Novus Biologicals (Littleton, Colorado, USA). .. IKKα inhibitor BAY 11–7082 (ab141228) was purchased from Abcam (Cambridge, UK).

    Article Title: Transport of maternal cholesterol to the fetus is affected by maternal plasma cholesterol concentrations in the Golden Syrian hamster
    Article Snippet: Protein concentrations were determined by the Lowry assay, equal amounts of protein pooled from three to four samples, and proteins separated on a Bio-Rad 4-15% Tris-HCl Ready gel (Bio-Rad Laboratories, Hercules, CA) under denaturing conditions. .. Proteins were transferred to a polyvinylidene difluoride membrane that was blocked in Tris-buffered saline containing 0.1% Tween and 5% dry milk for 1 h. After blocking, blots were incubated with chicken anti-LDL receptor IgG (Novus Biologicals, Littleton, CO) or rabbit anti-SR-BI receptor IgG (Novus Biologicals). .. After 1 h at 37°C, the membranes were washed and then incubated with the appropriate secondary antibody conjugated with peroxidase (rabbit anti-chicken IgG and donkey anti-rabbit; GE Healthcare).

    Article Title: Macrophage MST1 protects against schistosomiasis-induced liver fibrosis by promoting the PPARγ-CD36 pathway and suppressing NF-κB signaling
    Article Snippet: MST1 kinase inhibitor XMU-XP-1 (SML2233) was purchased from Sigma-Aldrich (St. Louis, MO, USA). .. The CD36 blocking antibody SR-BI (NB400-104) was purchased from Novus Biologicals (Littleton, Colorado, USA). .. IKKα inhibitor BAY 11–7082 (ab141228) was purchased from Abcam (Cambridge, UK).

    Incubation:

    Article Title: Transport of maternal cholesterol to the fetus is affected by maternal plasma cholesterol concentrations in the Golden Syrian hamster
    Article Snippet: Protein concentrations were determined by the Lowry assay, equal amounts of protein pooled from three to four samples, and proteins separated on a Bio-Rad 4-15% Tris-HCl Ready gel (Bio-Rad Laboratories, Hercules, CA) under denaturing conditions. .. Proteins were transferred to a polyvinylidene difluoride membrane that was blocked in Tris-buffered saline containing 0.1% Tween and 5% dry milk for 1 h. After blocking, blots were incubated with chicken anti-LDL receptor IgG (Novus Biologicals, Littleton, CO) or rabbit anti-SR-BI receptor IgG (Novus Biologicals). .. After 1 h at 37°C, the membranes were washed and then incubated with the appropriate secondary antibody conjugated with peroxidase (rabbit anti-chicken IgG and donkey anti-rabbit; GE Healthcare).

    Article Title: Transport of maternal cholesterol to the fetus is affected by maternal plasma cholesterol concentrations in the Golden Syrian hamster
    Article Snippet: Protein concentrations were determined by the Lowry assay, equal amounts of protein pooled from three to four samples, and proteins separated on a Bio-Rad 4-15% Tris-HCl Ready gel (Bio-Rad Laboratories, Hercules, CA) under denaturing conditions. .. Proteins were transferred to a polyvinylidene difluoride membrane that was blocked in Tris-buffered saline containing 0.1% Tween and 5% dry milk for 1 h. After blocking, blots were incubated with chicken anti-LDL receptor IgG (Novus Biologicals, Littleton, CO) or rabbit anti-SR-BI receptor IgG (Novus Biologicals). .. After 1 h at 37°C, the membranes were washed and then incubated with the appropriate secondary antibody conjugated with peroxidase (rabbit anti-chicken IgG and donkey anti-rabbit; GE Healthcare).



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    cd36 blocking antibody sr bi  (Novus Biologicals)
    93
    Novus Biologicals cd36 blocking antibody sr bi
    PEMs and liver tissues were collected from Mst1 +/+ and Mst1 ΔM/ΔM mice infected with 20 ± 5 cercariae and sacrificed at week 12. (A) RNA-seq analysis was performed on differentially expressed genes (DEG) in PEMs from infected Mst1 +/+ and Mst1 ΔM/ΔM mice control Mst1 +/+ and Mst1 ΔM/ΔM mice (n = 3). DEGs were identified in all pairwise comparisons and showed a 2-fold change in expression with an adjusted p- value of 0.05. (B) Some DEGs were verified by qPCR in PEMs from infected mice. (C) Liver sections from infected mice were subjected to HE staining (leftmost panel) and dual immunofluorescence for <t>CD36</t> and F4/80 (2–5 panels on the right side) in. High-magnification images of co-staining for F4/80 and CD36 cells were shown in the right most panel. The fluorescence intensity of CD36 overlaid with F4/80 + cells was analyzed using ImageJ. Data are shown as mean ± SEM of three different liver sections in each mouse (n = 9, data pooled from 3 independent experiments, 3 mice per group for each experiment. Bar of the left five panels = 100 μm, bar of the far right panel = 50 μm).All p values were determined by Student’s t -test. * P < 0.05, ** P < 0.01.
    Cd36 Blocking Antibody Sr Bi, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd36+blocking+antibody+sr-bi/SR-BI+Antibody+-+BSA+Free/pmc11785294-181-1-9
    Average 93 stars, based on 1 article reviews
    cd36 blocking antibody sr bi - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    PEMs and liver tissues were collected from Mst1 +/+ and Mst1 ΔM/ΔM mice infected with 20 ± 5 cercariae and sacrificed at week 12. (A) RNA-seq analysis was performed on differentially expressed genes (DEG) in PEMs from infected Mst1 +/+ and Mst1 ΔM/ΔM mice control Mst1 +/+ and Mst1 ΔM/ΔM mice (n = 3). DEGs were identified in all pairwise comparisons and showed a 2-fold change in expression with an adjusted p- value of 0.05. (B) Some DEGs were verified by qPCR in PEMs from infected mice. (C) Liver sections from infected mice were subjected to HE staining (leftmost panel) and dual immunofluorescence for CD36 and F4/80 (2–5 panels on the right side) in. High-magnification images of co-staining for F4/80 and CD36 cells were shown in the right most panel. The fluorescence intensity of CD36 overlaid with F4/80 + cells was analyzed using ImageJ. Data are shown as mean ± SEM of three different liver sections in each mouse (n = 9, data pooled from 3 independent experiments, 3 mice per group for each experiment. Bar of the left five panels = 100 μm, bar of the far right panel = 50 μm).All p values were determined by Student’s t -test. * P < 0.05, ** P < 0.01.

    Journal: PLOS Pathogens

    Article Title: Macrophage MST1 protects against schistosomiasis-induced liver fibrosis by promoting the PPARγ-CD36 pathway and suppressing NF-κB signaling

    doi: 10.1371/journal.ppat.1012790

    Figure Lengend Snippet: PEMs and liver tissues were collected from Mst1 +/+ and Mst1 ΔM/ΔM mice infected with 20 ± 5 cercariae and sacrificed at week 12. (A) RNA-seq analysis was performed on differentially expressed genes (DEG) in PEMs from infected Mst1 +/+ and Mst1 ΔM/ΔM mice control Mst1 +/+ and Mst1 ΔM/ΔM mice (n = 3). DEGs were identified in all pairwise comparisons and showed a 2-fold change in expression with an adjusted p- value of 0.05. (B) Some DEGs were verified by qPCR in PEMs from infected mice. (C) Liver sections from infected mice were subjected to HE staining (leftmost panel) and dual immunofluorescence for CD36 and F4/80 (2–5 panels on the right side) in. High-magnification images of co-staining for F4/80 and CD36 cells were shown in the right most panel. The fluorescence intensity of CD36 overlaid with F4/80 + cells was analyzed using ImageJ. Data are shown as mean ± SEM of three different liver sections in each mouse (n = 9, data pooled from 3 independent experiments, 3 mice per group for each experiment. Bar of the left five panels = 100 μm, bar of the far right panel = 50 μm).All p values were determined by Student’s t -test. * P < 0.05, ** P < 0.01.

    Article Snippet: The CD36 blocking antibody SR-BI (NB400-104) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Techniques: Infection, RNA Sequencing, Control, Expressing, Staining, Immunofluorescence, Fluorescence

    WT or Mst1 deficiency peritoneal exudate macrophages (PEMs) were stimulated with or without SEA (50μg/ml) for 24h, followed by incubation with FITC-Dextran for 2 h. (A) Flow cytometry analysis of the proportion of CD36 + cells. Isotype-matched control IgG staining (----); anti-mouse CD36 staining (——, no SEA;——, with SEA) The representative percentage of CD36 positive cells are shown in the left panels. Gating strategies are shown in the left panels. (B) Flow cytometry analysis of the proportion of PEMs engulfing FITC-dextran, with analysis of mean fluorescence intensity (MFI) in engulfed PEMs. (C) Immunofluorescence assay of PEMs engulfing FITC-dextran, with representative images from three separate cell isolates (magnification×200) and percentages of FITC + in F4/80 + PEMs shown. Ten viewing fields were analyzed in each experiment(Bar of the left three panels = 40 μm, bar of the far right panel = 20 μm). (D, E) Changes in gene expression in PEMs after the uptake of FITC-dextran (37°C, 2h). (F, G) SEA cultured WT PEMs were pretreated with isotype control (clone EPR25A, ab172730) and or CD36 function-blocking antibody (clone EPR22509-40, ab252922), followed by incubation with FITC-Dextran (2 h, 37°C), and subjected to immunofluorescent staining analysis. Representative images were shown (magnification×200) on left panels. Percentages of coimmunostaining of CD36 + and FITC + PEMs were shown on the right side (Bar of the left three panels = 40 μm, bar of the far right panel = 20 μm) (F). mRNA expressions of indicated genes were detected by qPCR (G). Data are shown as mean ± SEM (n = 3, three samples consisting of pooled PEMs of two or three mice from representative of three independent experiments). Statistical significance was determined by student t test (A-F) and one-way ANOVA analysis (G). * P < 0.05, ** P < 0.005, *** P < 0.001.

    Journal: PLOS Pathogens

    Article Title: Macrophage MST1 protects against schistosomiasis-induced liver fibrosis by promoting the PPARγ-CD36 pathway and suppressing NF-κB signaling

    doi: 10.1371/journal.ppat.1012790

    Figure Lengend Snippet: WT or Mst1 deficiency peritoneal exudate macrophages (PEMs) were stimulated with or without SEA (50μg/ml) for 24h, followed by incubation with FITC-Dextran for 2 h. (A) Flow cytometry analysis of the proportion of CD36 + cells. Isotype-matched control IgG staining (----); anti-mouse CD36 staining (——, no SEA;——, with SEA) The representative percentage of CD36 positive cells are shown in the left panels. Gating strategies are shown in the left panels. (B) Flow cytometry analysis of the proportion of PEMs engulfing FITC-dextran, with analysis of mean fluorescence intensity (MFI) in engulfed PEMs. (C) Immunofluorescence assay of PEMs engulfing FITC-dextran, with representative images from three separate cell isolates (magnification×200) and percentages of FITC + in F4/80 + PEMs shown. Ten viewing fields were analyzed in each experiment(Bar of the left three panels = 40 μm, bar of the far right panel = 20 μm). (D, E) Changes in gene expression in PEMs after the uptake of FITC-dextran (37°C, 2h). (F, G) SEA cultured WT PEMs were pretreated with isotype control (clone EPR25A, ab172730) and or CD36 function-blocking antibody (clone EPR22509-40, ab252922), followed by incubation with FITC-Dextran (2 h, 37°C), and subjected to immunofluorescent staining analysis. Representative images were shown (magnification×200) on left panels. Percentages of coimmunostaining of CD36 + and FITC + PEMs were shown on the right side (Bar of the left three panels = 40 μm, bar of the far right panel = 20 μm) (F). mRNA expressions of indicated genes were detected by qPCR (G). Data are shown as mean ± SEM (n = 3, three samples consisting of pooled PEMs of two or three mice from representative of three independent experiments). Statistical significance was determined by student t test (A-F) and one-way ANOVA analysis (G). * P < 0.05, ** P < 0.005, *** P < 0.001.

    Article Snippet: The CD36 blocking antibody SR-BI (NB400-104) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Techniques: Incubation, Flow Cytometry, Control, Staining, Fluorescence, Immunofluorescence, Gene Expression, Cell Culture, Blocking Assay

    (A) Pathway analysis of the RNA-Seq data comparing PEMs from Mst1 +/+ and Mst1 ΔM/ΔM mice infected by S . japonicum for 12 weeks. (B) Immunofluorescence assay of SEA (24 h, 50μg/ml) or control cultured WT and MST1- deficiency PEMs for CD36 and PPARγ. Images were collected with a confocal microscope and CD36 intensity was quantified using ImageJ. Data are shown as mean ± SEM (n = 3, three samples consisting of pooled PEMs of two or three mice from representative of three independent experiments. Bar = 10 μm). (C) Immunofluorescence assay for CD36 (green), PPARγ (red) in RAW264.7 cells stimulated with or without SEA (24 h, 50μg/ml), followed by XMU-MP-1 incubation (3μM, 6h). Images were taken with a confocal microscope. Percent nuclear PPARγ fluorescence and CD36 intensity in macrophages with PPARγ localization were analyzed using ImageJ. Data from at least three independent experiments, with 10 viewing fields were analyzed in each (Bar of the left four panels = 10 μm, bar of the far right panel = 5 μm). (D, E) Immunoblot analysis of phosphorylated (p-)PPARγ and total PPARγ (left and middle) and relative Cd36 mRNA (right) in RAW264.7 cells treated with SEA ± XMU-MP-1(D) and in liver tissues from Mst1 +/+ and Mst1 ΔM/ΔM mice infected and control mice (E). Values are means ± SEM (n = 3, from representative of three independent experiments). Statistical significance was determined by Mann-Whitney U-test (B), one way ANOVA analysis (C) and Student’s t test (D-E). * P < 0. 05, ** P < 0. 01.

    Journal: PLOS Pathogens

    Article Title: Macrophage MST1 protects against schistosomiasis-induced liver fibrosis by promoting the PPARγ-CD36 pathway and suppressing NF-κB signaling

    doi: 10.1371/journal.ppat.1012790

    Figure Lengend Snippet: (A) Pathway analysis of the RNA-Seq data comparing PEMs from Mst1 +/+ and Mst1 ΔM/ΔM mice infected by S . japonicum for 12 weeks. (B) Immunofluorescence assay of SEA (24 h, 50μg/ml) or control cultured WT and MST1- deficiency PEMs for CD36 and PPARγ. Images were collected with a confocal microscope and CD36 intensity was quantified using ImageJ. Data are shown as mean ± SEM (n = 3, three samples consisting of pooled PEMs of two or three mice from representative of three independent experiments. Bar = 10 μm). (C) Immunofluorescence assay for CD36 (green), PPARγ (red) in RAW264.7 cells stimulated with or without SEA (24 h, 50μg/ml), followed by XMU-MP-1 incubation (3μM, 6h). Images were taken with a confocal microscope. Percent nuclear PPARγ fluorescence and CD36 intensity in macrophages with PPARγ localization were analyzed using ImageJ. Data from at least three independent experiments, with 10 viewing fields were analyzed in each (Bar of the left four panels = 10 μm, bar of the far right panel = 5 μm). (D, E) Immunoblot analysis of phosphorylated (p-)PPARγ and total PPARγ (left and middle) and relative Cd36 mRNA (right) in RAW264.7 cells treated with SEA ± XMU-MP-1(D) and in liver tissues from Mst1 +/+ and Mst1 ΔM/ΔM mice infected and control mice (E). Values are means ± SEM (n = 3, from representative of three independent experiments). Statistical significance was determined by Mann-Whitney U-test (B), one way ANOVA analysis (C) and Student’s t test (D-E). * P < 0. 05, ** P < 0. 01.

    Article Snippet: The CD36 blocking antibody SR-BI (NB400-104) was purchased from Novus Biologicals (Littleton, Colorado, USA).

    Techniques: RNA Sequencing, Infection, Immunofluorescence, Control, Cell Culture, Microscopy, Incubation, Fluorescence, Western Blot, MANN-WHITNEY